FastQCFastQC Report
Sat 31 Dec 2022
SRR1954277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712136
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16480.23141647101115517No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14600.20501701922104767No Hit
GAGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT10200.1432310682229237No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC9110.12792500308929755Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8260.11598908073738724No Hit
AAGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT7250.1018063965309997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)