FastQCFastQC Report
Sat 31 Dec 2022
SRR1954275.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954275.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123880
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24580.21870662348293413No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21620.19236929209524148No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC16270.14476634516140513TruSeq Adapter, Index 16 (95% over 22bp)
GAGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT16090.14316475068512652No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT15470.13764814748905577TruSeq Adapter, Index 13 (95% over 22bp)
AAGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT14230.12661494109691426No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13180.11727230665195573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)