FastQCFastQC Report
Sat 31 Dec 2022
SRR1954274.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954274.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939611
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG212672.263383464007978No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC209982.234754595252716No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC164031.7457224319425806No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCT38940.4144268213122239No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTC38500.4097440323708428No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT33580.35738193784449096No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28160.2996984922483879No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC26940.28671439563819495No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTA20490.21806896683840438No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTT15460.1645361750767073No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGT9860.10493704309549377No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACAAGT9590.10206351351782814No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)