FastQCFastQC Report
Sat 31 Dec 2022
SRR1954267.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954267.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601740
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21450.35646624788114467No Hit
GAGCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCT11830.19659653671020705RNA PCR Primer, Index 43 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.19011533220327717No Hit
AAGCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCT10100.16784657825638982RNA PCR Primer, Index 43 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8160.13560674045268722No Hit
CTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGCT7650.12713131917439427RNA PCR Primer, Index 24 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC7360.12231196197693356RNA PCR Primer, Index 43 (96% over 25bp)
AAGAGCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTT6390.10619204307508226RNA PCR Primer, Index 43 (95% over 21bp)
AGAAATGGGACCTTCTGTCATTGTGATGAGAAAGTAAGTTCATTTAACTTA6310.1048625652275069No Hit
TAGAGCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTT6070.10087413168478081No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)