FastQCFastQC Report
Sat 31 Dec 2022
SRR1954262.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954262.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882107
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23770.2694684431707265No Hit
GAGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT12450.1411393402387692No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC12450.1411393402387692TruSeq Adapter, Index 14 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12000.13603791830242817No Hit
AAGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT10900.12356777579137226No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT10670.12096038235724237Illumina Paired End PCR Primer 2 (95% over 21bp)
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9900.11223128259950324No Hit
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC9410.10667640093548741TruSeq Adapter, Index 14 (95% over 21bp)
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT8890.10078142447571554No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)