FastQCFastQC Report
Sat 31 Dec 2022
SRR1954254.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954254.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544959
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.31176657326514473No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11850.21744755110017452No Hit
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT6290.11542152712405887Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC5900.10826502544228098No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC5860.10753102526979094No Hit
GAGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT5800.10643002501105588No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)