FastQCFastQC Report
Sat 31 Dec 2022
SRR1954253.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954253.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520647
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14070.2702406813061441No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12360.23739693112607968No Hit
GAGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCT9390.18035252291859935No Hit
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC6770.13003051971873458No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTT6570.12618914542866855No Hit
AAGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCT5870.11274433541343751No Hit
CTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGCT5290.10160434997224607Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)