FastQCFastQC Report
Sat 31 Dec 2022
SRR1954243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464816
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15770.3392740353172008No Hit
GAGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCT15570.33497125744380574No Hit
AAGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCT13130.2824773673883859No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.25666070014801556No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC10830.2329954218443427TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT9060.1949158376647964TruSeq Adapter, Index 16 (95% over 23bp)
AAGAGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTT7650.1645812536573612No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC7640.16436611476369145TruSeq Adapter, Index 13 (95% over 21bp)
TAGAGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTT6810.14650958658910193No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTT6360.13682833637396302No Hit
AGAGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC5980.1286530584145124No Hit
AGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTG5180.11144194692093215TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC4840.10412722453616054TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)