FastQCFastQC Report
Sat 31 Dec 2022
SRR1954241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521231
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16210.3109945494416103No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13640.2616881958287208No Hit
GAGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT7630.14638423271064077No Hit
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC7300.14005306668252657TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT7210.1383263850384954TruSeq Adapter, Index 15 (95% over 22bp)
AAGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT6550.12566405298226696No Hit
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC5410.10379275215787241TruSeq Adapter, Index 21 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5330.10225792402984474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)