FastQCFastQC Report
Sat 31 Dec 2022
SRR1954232.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954232.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427598
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14670.3430792473304365No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12110.2832099308228757No Hit
AAGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCT6410.14990715578650976No Hit
GAGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCT5970.13961711701177276No Hit
CTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGCT5660.1323673169659353Illumina Single End Adapter 1 (95% over 21bp)
AAGAGCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTT5080.11880317494469103No Hit
GCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC4600.10757767809952339No Hit
CCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC4410.10313425226497784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)