FastQCFastQC Report
Sat 31 Dec 2022
SRR1954230.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954230.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419233
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12830.30603506880422104No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10830.25832890063520764No Hit
GAGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT8760.20895301658027873No Hit
AAGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT8460.20179709135492674No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGCT7390.17627429138450454Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC6940.16554040354647653No Hit
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC6620.1579074166394344No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT6120.14598087459718107No Hit
TAGAGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT4370.1042379774492943No Hit
AGGACAGGGGAAAGTAAAAAAACAAAGGCGGGGGAATCAATACAATCAGTA4260.10161413819999857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)