FastQCFastQC Report
Sat 31 Dec 2022
SRR1954228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674792
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16330.24200049793121434No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12450.18450129817780886No Hit
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC9250.1370792777626291Illumina PCR Primer Index 8 (95% over 22bp)
GAGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT8370.12403822214845464No Hit
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT7230.10714412737554684RNA PCR Primer, Index 29 (96% over 26bp)
AAGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT7120.10551399542377503TruSeq Adapter, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)