FastQCFastQC Report
Sat 31 Dec 2022
SRR1954227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043393
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94050.9013861507600683No Hit
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT12420.11903472612908081Illumina Single End Adapter 1 (95% over 21bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10840.10389182216096907No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTT10740.1029334105174177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)