FastQCFastQC Report
Sat 31 Dec 2022
SRR1954226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807844
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23060.2854511514599353No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21480.2658929199201826No Hit
GAGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT17950.2221963646446591Illumina PCR Primer Index 11 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT15920.1970677507043439Illumina PCR Primer Index 11 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC15720.1945920251929828Illumina PCR Primer Index 11 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT12790.15832264645154262Illumina PCR Primer Index 11 (95% over 24bp)
TAGAGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT10240.12675714618168854No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT10230.12663335990612049No Hit
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC9760.12081540495442189Illumina PCR Primer Index 11 (95% over 23bp)
GAGAGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT9300.11512123627829135No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)