FastQCFastQC Report
Sat 31 Dec 2022
SRR1954225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620989
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.3061245851375789No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15300.2463811758340325No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC14630.23559193480077747TruSeq Adapter, Index 16 (95% over 21bp)
GAGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT14100.22705716204312795No Hit
AAGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT12610.20306317825275486No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT11780.18969740204737925TruSeq Adapter, Index 13 (95% over 22bp)
AAGAGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTT9860.15877897998193205No Hit
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC8750.14090426722534538TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC8000.12882675860603007No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG7560.1217412868826984No Hit
TAGAGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTT7480.1204530192966381No Hit
GAGAGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTT7310.11771545067625996No Hit
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC6910.11127411274595846TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)