FastQCFastQC Report
Sat 31 Dec 2022
SRR1954220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610788
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.2277385934235774No Hit
GAGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT11040.18075011296882063No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC10960.17944032954151032TruSeq Adapter, Index 14 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9520.155864227849925No Hit
AAGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT9230.1511162629259252No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT7950.13015972808896048Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC7610.12459314852289174TruSeq Adapter, Index 14 (95% over 21bp)
GAGAGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTT7470.12230102752509872No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTT7170.11738933967268513No Hit
TAGAGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTT7080.11591583331696104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)