FastQCFastQC Report
Sat 31 Dec 2022
SRR1954208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549935
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15430.28057861383618066No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11140.2025693945648122No Hit
GAGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT6800.12365097693363761No Hit
AAGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT6410.11655922972714956No Hit
AAGAGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTT6050.11001300153654524No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT5850.1063762080973206Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)