FastQCFastQC Report
Sat 31 Dec 2022
SRR1954207.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954207.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507563
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15710.30951822729395173No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11810.23268047513313617No Hit
GAGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT11550.22755795832241515TruSeq Adapter, Index 19 (95% over 21bp)
GAGAGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT8920.17574173058319853No Hit
AAGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT8620.1698311342631358TruSeq Adapter, Index 13 (95% over 21bp)
AAGAGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT7830.15426656395363728No Hit
TAGAGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT7140.140672192417493No Hit
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC6370.12550166186266531TruSeq Adapter, Index 19 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)