FastQCFastQC Report
Sat 31 Dec 2022
SRR1954205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548379
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60281.099239759363506No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTT7690.14023148224129664No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.1205370738120898No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCT6210.11324284846793914No Hit
CGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTG6010.1095957357958638Illumina PCR Primer Index 7 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC5830.10631333439099601No Hit
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT5810.10594862312378849Illumina PCR Primer Index 7 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)