FastQCFastQC Report
Sat 31 Dec 2022
SRR1954200.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954200.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718411
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20890.2907806255750538No Hit
GAGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT7680.10690259475425627TruSeq Adapter, Index 13 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7600.10578902605889944No Hit
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC7580.10551063388506021TruSeq Adapter, Index 13 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT7470.10397947692894456Illumina PCR Primer Index 4 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)