FastQCFastQC Report
Sat 31 Dec 2022
SRR1954198.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954198.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367293
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18650.5077690018595508No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10480.2853307849591471No Hit
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT8710.23714037566738272TruSeq Adapter, Index 3 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC7760.21127546672547529RNA PCR Primer, Index 3 (95% over 23bp)
GAGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT7120.19385068596461136RNA PCR Primer, Index 24 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5300.1442989656759045No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC5170.14075955708385401RNA PCR Primer, Index 24 (95% over 23bp)
AAGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT4650.12660192271565207TruSeq Adapter, Index 3 (95% over 21bp)
TACATGGGAAGAACTTCAGAAGTTAAATAAAAAAAGAGAGAATGAGAAAAG4410.1200676299303281No Hit
AGGATAGGGATGTTTGGGTCGTTGCTAATCTCATCTAAGATGTGGATTGCT3900.10618225776151465No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC3890.10590999556212616RNA PCR Primer, Index 24 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)