FastQCFastQC Report
Sat 31 Dec 2022
SRR1954194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947358
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24550.2591417394480228No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC16300.1720574481874856Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT15730.16604071533675757TruSeq Adapter, Index 27 (95% over 24bp)
GAGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT13190.1392293093001801No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC12310.12994031823238944Illumina Single End Adapter 1 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT11470.12107355403131656TruSeq Adapter, Index 27 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11320.11949020328112499No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)