FastQCFastQC Report
Sat 31 Dec 2022
SRR1954192.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954192.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583861
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.2891099080089268No Hit
GAGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT8740.14969316327002488No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8630.14780915320598567No Hit
GCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGC6880.11783626582354363TruSeq Adapter, Index 22 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT6590.1128693302001675No Hit
CTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGCT6020.10310673259560066TruSeq Adapter, Index 20 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)