FastQCFastQC Report
Sat 31 Dec 2022
SRR1954190.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954190.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870192
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18200.20914924522404252No Hit
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC12070.13870502142056007TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT11670.13410833471233935Illumina PCR Primer Index 5 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC10320.11859451707209444TruSeq Adapter, Index 23 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph