FastQCFastQC Report
Sat 31 Dec 2022
SRR1954183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424533
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.30527662160538754No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8160.19221120619598478No Hit
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT6620.155936052085468Illumina PCR Primer Index 5 (96% over 26bp)
GAGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT6590.15522939323915927RNA PCR Primer, Index 33 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC6140.14462951054452775RNA PCR Primer, Index 33 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC5650.1330874160548179Illumina PCR Primer Index 5 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)