FastQCFastQC Report
Sat 31 Dec 2022
SRR1954182.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954182.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474900
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.40134765213729207No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13390.28195409559907353No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC9960.20972836386607707TruSeq Adapter, Index 22 (95% over 23bp)
GAGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCT9600.20214782059380923TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT8960.1886712992208886TruSeq Adapter, Index 20 (95% over 22bp)
AAGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCT8330.17540534849441988No Hit
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC6490.1366603495472731TruSeq Adapter, Index 22 (95% over 23bp)
TAGAGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTT5930.12486839334596757No Hit
AAGAGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTT5850.12318382817435249No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5430.11433986102337335No Hit
TCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC5370.11307643714466202TruSeq Adapter, Index 22 (95% over 23bp)
GAGAGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTT5200.10949673615498No Hit
GGAATAGGGAAACCACACCCATCAGCCATTCTGGAAGTGCACTATTTATAC4970.10465361128658664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)