FastQCFastQC Report
Sat 31 Dec 2022
SRR1954180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549365
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.308902096056356No Hit
GAGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT13680.24901477159993812TruSeq Adapter, Index 16 (95% over 22bp)
AAGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT13680.24901477159993812TruSeq Adapter, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT10990.20004914765228946TruSeq Adapter, Index 27 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10840.19731872252509713No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC10800.19659060915784587TruSeq Adapter, Index 16 (95% over 24bp)
AAGAGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT10260.1867610786999536No Hit
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC9640.17547532150755873TruSeq Adapter, Index 16 (95% over 24bp)
TAGAGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT6980.12705578258534855No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6380.11613408207657933No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT6060.11030917513856907No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)