FastQCFastQC Report
Sat 31 Dec 2022
SRR1954174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598944
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24450.40821846449751564No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9280.15493935994016134No Hit
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT6970.11637148047229792Illumina PCR Primer Index 7 (96% over 28bp)
GAGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT6590.11002698081957578Illumina PCR Primer Index 7 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6380.10652080995886093No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)