FastQCFastQC Report
Sat 31 Dec 2022
SRR1954102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557231
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGAAACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG21020.37722237276820564No Hit
GAATCTATCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC20440.36681376305338365No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG18200.32661499449958814No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC17180.3083101981045563No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC16890.30310589324714526No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13450.24137207011095937No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC13340.23939802344090688No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG12950.23239913070163No Hit
GAATATGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC11160.20027600761623096No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC8020.1439259481256427No Hit
GCTGTCACTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC6340.11377687171029609TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)