FastQCFastQC Report
Sat 31 Dec 2022
SRR1954101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666734
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGAAACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG18480.27717200562743166No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17310.2596237779984222No Hit
GAATCTATCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC14270.21402838313330352No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG14240.21357842857871356No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGC13490.20232956471396388No Hit
GAATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC10410.15613423044272529No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGC10300.15448439707589534No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG8550.12823704805814612No Hit
GCTGTCACTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC8220.12328754795765627No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7270.1090389870623067No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6740.10108978993121694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)