FastQCFastQC Report
Sat 31 Dec 2022
SRR1954068.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954068.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664451
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTATTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT7690.11573464408963188TruSeq Adapter, Index 12 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7150.1076076339715043No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.10580163172303149No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)