FastQCFastQC Report
Sat 31 Dec 2022
SRR1954066.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954066.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641955
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTATTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT9400.14642770910733618Illumina PCR Primer Index 11 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8980.1398851944450935No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8160.1271117134378578No Hit
GAGCTGACTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT7950.12384045610673644Illumina PCR Primer Index 11 (95% over 21bp)
GCTGTCACTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC7060.10997655598912695Illumina PCR Primer Index 11 (95% over 23bp)
AAGCTGACTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT6800.1059264278648815Illumina PCR Primer Index 11 (95% over 21bp)
CCTGTCACTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC6530.10172052558201121Illumina PCR Primer Index 11 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)