FastQCFastQC Report
Sat 31 Dec 2022
SRR1954039.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954039.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84226
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1390.1650321753377817No Hit
GAGCTGACTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT1320.15672120247904447Illumina PCR Primer Index 10 (95% over 21bp)
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.14247382043549497No Hit
CTGTCTATTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT930.1104172108375086Illumina PCR Primer Index 10 (95% over 24bp)
AAGAGCAGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT900.10685536532662122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)