FastQCFastQC Report
Sat 31 Dec 2022
SRR1953979.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953979.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68197
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1620.2375471061776911No Hit
GAGCTGACTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT1100.16129741777497544No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.12170623341202694No Hit
CTGTCTATTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT690.10117747114975731Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)