FastQCFastQC Report
Sat 31 Dec 2022
SRR1953947.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953947.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49842
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1700.34107780586653824No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.18859596324385056No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.15850086272621483No Hit
CCTGTCACTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC520.10432968179447052TruSeq Adapter, Index 19 (95% over 21bp)
GAGCTGACTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT500.10031700172545242No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)