FastQCFastQC Report
Sat 31 Dec 2022
SRR1953856.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953856.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523034
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA24410.4667000615638754No Hit
AAGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA14880.28449393347277613No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13710.26212445080052155No Hit
CACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTCGCAGTCAGGC12810.24491715643724885No Hit
GTGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA10210.19520719494334976No Hit
GTGCAAGGGGTACGCTGTCAGGCCATAAAAGCCCTCCAACTGCTTGTAGGC9290.17761751626089317No Hit
AGGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA8530.1630869121319073No Hit
GGGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA7020.13421689603352746No Hit
AGGCCTGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGAT6680.1277163626074022No Hit
TGGCCTGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGAT6270.11987748406413351No Hit
GTGCACGGGGTCTTTCCGTCTAACCGCGGGTACTCCGCATCTTCACGGAGA6010.11490648791474359No Hit
GCGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA5850.1118474133612729No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)