FastQCFastQC Report
Sat 31 Dec 2022
SRR1953850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486393
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA18260.3754165869985793No Hit
CACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTCGCAGTCAGGC13740.2824876180372662No Hit
GTGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA10660.21916433830256604No Hit
GTGCAAGGGGTACGCTGTCAGGCCATAAAAGCCCTCCAACTGCTTGTAGGC10170.20909018016295466No Hit
AGGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA8910.18318520208966824No Hit
AAGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA8180.16817676241228802No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6870.1412438090186331No Hit
GCGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA6860.14103821395455937No Hit
AGGCCTGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGAT6090.12520739402088435No Hit
GGGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA5600.11513323588127297No Hit
TGGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA5570.11451645068905186No Hit
GTGCACGGGGTCTTTCCGTCTAACCGCGGGTACTCCGCATCTTCACGGAGA5040.10361991229314567No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)