FastQCFastQC Report
Sat 31 Dec 2022
SRR1953839.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953839.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546159
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA22810.4176439461768459No Hit
CACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTCGCAGTCAGGC13190.24150476326491002No Hit
AAGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA12440.22777249848487344No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG10040.1838292511887564No Hit
AGGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA9960.18236447627888583No Hit
GTGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA8630.1580125934022876No Hit
GTGCAAGGGGTACGCTGTCAGGCCATAAAAGCCCTCCAACTGCTTGTAGGC8000.1464774909870569No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC7440.1362240666179629No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6100.11168908687763086No Hit
GCGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA6050.11077360255896176No Hit
GTGCACGGGGTCTTTCCGTCTAACCGCGGGTACTCCGCATCTTCACGGAGA5820.1065623746930839No Hit
ACGATTGGGGGCCTTAGCTGTAGGTCAGGGCTGTTTCCCTTTTGACGACGG5570.10198495309973836No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC5530.10125256564480307No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)