FastQCFastQC Report
Sat 31 Dec 2022
SRR1953837.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953837.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749942
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA36650.48870445981155874No Hit
CACTATGACCGACTTTCGTCTCTGCTCGACTTGTCAGTCTCGCAGTCAGGC22730.3030901056348358No Hit
AAGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA21850.2913558648535487No Hit
GTGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA17910.23881846862824058No Hit
GTGCAAGGGGTACGCTGTCAGGCCATAAAAGCCCTCCAACTGCTTGTAGGC17780.2370850012400959No Hit
AGGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA15600.20801608657736195No Hit
GCGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA14300.19068141269591515No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13400.17868048462414426No Hit
GGGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA10400.13867739105157464No Hit
GCGATTGGGGGCCTTAGCTGTAGGTCAGGGCTGTTTCCCTTTTGACGACGG9470.12627643204407807No Hit
GTGCACGGGGTCTTTCCGTCTAACCGCGGGTACTCCGCATCTTCACGGAGA8870.11827581332956416No Hit
TGGCCTGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGAT8530.1137421293913396No Hit
AGGCTTGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGAT8350.11134194377698542No Hit
TGGATAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA8030.10707494712924466No Hit
GTACCTGGGCTTCATTTCAGAGCTTGCACCCCTCCACTTAACCTTCAGGCA7970.10627488525779326No Hit
ACGATTGGGGGCCTTAGCTGTAGGTCAGGGCTGTTTCCCTTTTGACGACGG7880.10507479245061618No Hit
AGGCGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGA7850.1046747615148905No Hit
GCAGAAGGGACCAAACTGTCTCGCGACGTTCTGAACCCAGCTCACGTACCA7730.10307463777198769No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)