FastQCFastQC Report
Sat 31 Dec 2022
SRR1953750.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953750.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574583
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19360.3369400069267625No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15310.2664541067173933No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14540.2530530837146243No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT11870.2065846013543735No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7260.12635250259753597No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT7160.12461210999977375No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG7080.12321979592156398No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6970.12130536406402556No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6720.11695438256962006No Hit
GGGCATGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6580.11451783293275296No Hit
AGTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6530.11364763663387187No Hit
AGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT6380.11103704773722857No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)