FastQCFastQC Report
Sat 31 Dec 2022
SRR1953745.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953745.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903171
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35000.38752351437324717No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21940.24292188300997264No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19580.2167917260408051No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18390.20361592655211472No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12800.1417228852565018No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT12430.13762620810455606No Hit
AGTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT10180.11271398218056161No Hit
GGGCATGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9840.10894946804093576No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9790.10839586302040255No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9620.10651360595058965No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9210.1019740447822173No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)