FastQCFastQC Report
Sat 31 Dec 2022
SRR1953743.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953743.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379365
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67280.48776067248335286No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57560.41729346474645945No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT50280.36451555607109065No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40650.2947008224799092No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33680.24417032475088177No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28070.20349943633483522No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG25610.18566514301870787No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24180.1752980538146176No Hit
GGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG23220.16833832959368986No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC22350.16203107951847406No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG20470.1484016195858239No Hit
AGTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20340.1474591569309066No Hit
AGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20030.14521174598456538No Hit
GGGCAGGGGCTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC19620.14223936376521082No Hit
GGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18960.137454553363323No Hit
GGGCATGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18370.13317722285254446No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18230.13216226307032586No Hit
AGGGTAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16010.11606790080943043No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC15620.11324051284467852No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG15400.11164557604404926No Hit
AAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT15210.11026813062532397No Hit
GGGGTAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14690.10649828000565478No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)