FastQCFastQC Report
Sat 31 Dec 2022
SRR1953742.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953742.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164139
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53140.45647469932714224No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30010.2577870855628065No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28300.2430981180082447No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21870.18786416398729017No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20180.17334699722284022No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18230.15659642018693642No Hit
GGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17580.15101289450830185No Hit
AGTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16110.13838553643508206No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT15980.13726883129935513No Hit
GGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG14710.1263594811272537No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14470.12429787164591169No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13920.11957334991783627No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)