FastQCFastQC Report
Sat 31 Dec 2022
SRR1953736.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953736.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382211
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG149591.082251552042344No Hit
GAATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC100860.729700458178961No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC87540.6333331162897705No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58220.42120920756671737No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43890.3175347323961392No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38720.28013089173794736No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38360.2775263689841855No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT31200.225725305326032No Hit
GAACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCT28200.20402094904468276No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTC27210.1968585114718375No Hit
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC25490.18441468053719728No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22370.1618421500045941No Hit
GGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20080.1452744913764975No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19980.14455101283378588No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18810.13608631388405967No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17730.12827274562277394No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCCCACTT17630.1275492670800623No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCCCACTTCGTA16370.11843343744189563No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16200.11720352391928583No Hit
GGGCAGGGGCTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC16070.1162630018137607No Hit
GGGCATGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT15940.11532247970823556No Hit
GGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG15890.11496074043687976No Hit
AGTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14880.10765360715549219No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)