FastQCFastQC Report
Sat 31 Dec 2022
SRR1953731.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953731.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159027
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48130.41526211209920044No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40740.3515017337818705No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35270.3043069747296655No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26790.23114215630869686No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25310.21837282479182968No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18890.1629815353740681No Hit
AGTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18740.1616873463689802No Hit
GGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG18230.15728710375168137No Hit
GGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT17850.1540084916054587No Hit
GTGAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC15510.13381914312608764No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14660.12648540543058964No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14050.1212223701432322No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC13680.11803003726401542TruSeq Adapter, Index 16 (95% over 21bp)
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13330.11501026291881034No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG12790.11035118250049396No Hit
GGGCATGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT11700.1009467423968553No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)