FastQCFastQC Report
Sat 31 Dec 2022
SRR1953728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168448
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG103080.8821958700772305No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC96730.8278502766062332RNA PCR Primer, Index 10 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80450.688520156652243No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC62180.532158897956948No Hit
GAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT29180.2497329791312921TruSeq Adapter, Index 10 (95% over 22bp)
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27880.23860710960179657No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27130.2321883387193953No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25360.21704003943692832No Hit
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC24740.2117338555074766RNA PCR Primer, Index 35 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT23300.19940981541326616No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTC20830.17827066330722463No Hit
GGGCAAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20400.17459056800131456No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18460.15798734731883662No Hit
AGTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT15960.13659144437749904No Hit
GGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG15390.13171317850687408No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14270.12212781398915486No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14150.12110081064797062No Hit
GGGCATGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13530.11579462671851894No Hit
GGAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13510.11562345949498823No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13220.11314153475379307No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTTCTCT12970.11100194445965932No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)