FastQCFastQC Report
Sat 31 Dec 2022
SRR1953631.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953631.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4628080
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471391.018543326822354No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT68700.14844168640127223TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT56610.12231854246253307No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC51520.11132046118476775TruSeq Adapter, Index 27 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)