FastQCFastQC Report
Sat 31 Dec 2022
SRR1953628.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953628.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196999
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221321.8489572672993042No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT19950.16666680590376434RNA PCR Primer, Index 41 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCT17230.14394331156500548RNA PCR Primer, Index 41 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC15170.1267336062937396Illumina PCR Primer Index 11 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)