FastQCFastQC Report
Sat 31 Dec 2022
SRR1953539.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953539.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235733
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135111.093359164156011No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC55120.44605104824424047RNA PCR Primer, Index 41 (95% over 23bp)
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53510.43302234382346344No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG32720.2647821171725607No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC21530.1742285752666636No Hit
GAATCTGACTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC21050.1703442410294133Illumina PCR Primer Index 11 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT19570.1583675437978916Illumina PCR Primer Index 11 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)