FastQCFastQC Report
Sat 31 Dec 2022
SRR1953505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975595
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCG94030.9638220778089268No Hit
GAATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC69470.7120782701838365No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGC56470.5788262547471031No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41480.42517643079351575No Hit
GAATCTGACTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC24450.2506162905713949No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24260.24866876111501188No Hit
GAACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT19690.20182555261148322No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15200.15580235651064223No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTAGACGT14920.15293231310123565No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14790.15159979294686832No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC14570.14934475883947745No Hit
GAATGACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCT13990.14339966891999242No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTC11710.1200293154433961No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)